Journal: Stem Cell Research & Therapy
Article Title: Apoptotic vesicles from endothelial cells promote endothelial progenitor cell differentiation and angiogenesis via miR-30a-5p mediated activation of the EGFR/PI3K/AKT/VEGF pathway
doi: 10.1186/s13287-026-04912-x
Figure Lengend Snippet: Isolation, Characterization and Functional Evaluation of Apo-EVs. A , B Flow cytometry analysis of apoptosis of ECs induced by STS. C The size distribution of Apo-EVs was examined by NTA and SEM. D Flow cytometry showed the positive ratio of Annexin V positive vesicles in Apo-EVs. E The expression of β-actin, histone H3, cleaved caspase3 and CD63 in ECs and Apo-EVs was examined by western blot. F , G The effect of Apo-EVs on the migration and invasion ability of EPCs was measured by Transwell assay. Flow cytometry H , I and Immunofluorescent staining ( J ) showed that DiD-labeled Apo-EVs were engulfed by EPCs. N = 3 per group. **** p < 0.0001
Article Snippet: Staurosporine (STS), Y-27,632, CHIR99021, bFGF, BMP4 and VEGF were purchased form MedChemExpress. histone H3, CD63, DAPI, and Phalloidin Peptide were purchased form Proteintech.
Techniques: Isolation, Functional Assay, Flow Cytometry, Expressing, Western Blot, Migration, Transwell Assay, Staining, Labeling